Mike Zeck's illustration of Marvel's Secret Wars #8 features the black Spider suit for the first time. The symbiote suit eventually led to the introduction of the Venom character.
The record auction, which started at $330,000 and went over $3 million, was held on the first day of the four-day Heritage Auctions event in Dallas.
The previous record for an American comic book page was $657,250 from the 1974 issue of The Incredible Hulk.
Also Thursday, one of the few surviving copies of Superman's debut comic, Action Comics No. 1 sold for $3.18 million, making it one of the most expensive books ever auctioned.